Fig. 3
BZBS rejuvenates energy metabolism in aged mice. NMDS plots of dark phase (A) and light phase (B) showing differences in energy metabolism in young, aged, BZBS-treated, and NMN-treated mice measured by indirect calorimetry. Fitted curves and statistical analysis of VO2 (C, D), VCO2 (E, F), and EE (G, H) in mice of each group. Amplitude values of EE (I), phase adjustment values of EE (J), and RER (K) in mice of each group. Random forest mathematical models constructed using energy metabolism parameters of mice of different age groups (L, M), and use of this mathematical model to predict the biological age of EE in young, aged, BZBS-treated, and NMN-treated mice (N). n = 4. Statistical significance test was performed using one-way ANOVA and Sidak multiple comparison test. Data are presented as mean ± standard deviation. *p < 0.05, **p < 0.01, ***p < 0.001