Fig. 6

Protodioscin enhanced the expression of p62 and P-p62. The effects of protodioscin on p62 mRNA expression as determined by qRT-PCR (A). The effect of protodioscin on the levels of p62 and P-p62 was quantified by WB (B). qRT-PCR and WB verified the mRNA and protein levels of p62 after p62 silencing (C, D). WB and qRT-PCR verified the protein and mRNA levels of HO-1 and NQO1 after p62 silencing (E–G). Expression of data was undertaken in form of mean ± standard deviation, and the experiments were replicated independently on a minimum of three occasions, * P < 0.05; *It is suggestive of statistical significance at the P threshold below 0.05; **Denotes heightened significance at the P threshold below 0.01; ***Reflects substantial significance at the P threshold below 0.001; ****Demonstrates remarkable significance at the P threshold below 0.0001