Fig. 9

In the context of evaluating the therapeutic influence of protodioscin on pulmonary mouse fibrosis, discernible alterations were noted in the levels of ROS and various oxidative stress markers, involving 8-OHdG, MDA, SOD, and GSH, within the pulmonary tissues of fibrotic mice. The levels of ROS in lung tissues of mice in each group were detected by DHE staining (A). Immunohistochemical methods were used to detect the expression level of 8-OHdG in mouse lung tissues (magnification × 100) (B). MDA and GSH levels as well as SOD activity were biochemically assessed (C–E). qRT-PCR and WB were used to assess the level of mRNA and protein expression for HO-1 and NQO1 (F–H). ML385 indicates the group treated with ML385 prior to high-dose treatment; BLM + H indicates the high-dose treatment group; BLM indicates the lung fibrosis model group; Control indicates the control group. Expression of data was undertaken in form of mean ± standard deviation. The sample size, particularly per group, was eight, * P < 0.05; *It is suggestive of statistical significance at the P threshold below 0.05; **Denotes heightened significance at the P threshold below 0.01; ***Reflects substantial significance at the P threshold below 0.001; ****Demonstrates remarkable significance at the P threshold below 0.0001