Effect of FZHY drug serum on apoptosis in hepatocytes in vitro. Primary hepatocytes were isolated from mice and cultured; apoptosis was induced by TNF-α and actinomycin D (Act D) for six hours. Control group was incubated with normal rat serum, while treatment group was administered with FZHY drug serum at the same time. Normal control was cultured with newborn calf serum without TNF-α and Act D. The cell apoptosis was checked with Annexin V/Propidium Iodide (PI) stain and observed under the confocal laser scanning microscopy. Early apoptotic cells were Annexin V positive (green) alone, late apoptotic and necrotic cells were both Annexin V and PI positive or PI positive (red) alone respectively. (×630).