Polysaccharopeptides derived from Coriolus versicolor potentiate the S-phase specific cytotoxicity of Camptothecin (CPT) on human leukemia HL-60 cells

Table 1 The effect of CPT and CPT with PSP pretreatment on normal human peripheral blood mononuclear cells.

Treatment	Viable cells	Apoptotic cells	Necrotic cells
Control	37.93 (5.75)	29.62 (1.87)	31.51 (5.00)
PSP (25 μg/ml)	39.33 (4.70)	28.26 (2.18)	31.49 (4.11)
CPT (1 μM)	34.91 (5.39)	30.95 (2.49)	33.33 (5.05)
PSP (25 μg/ml) + CPT (1 μM)	40.80 (9.26)	28.85 (4.51)	29.38 (6.52)

Normal human peripheral blood mononuclear cells were treated with PSP (25 μg/ml) for 72 hour prior to addition of CPT (1 μM). CPT was added at 72 hours and samples were collected after five hours of drug incubation. Cells were identified and quantified using Annexin V/PI flow cytometric assay. Values are expressed as mean (SD) (n = 8).

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