Figure 7

Gene expression bioresponse profiles. Composite union gene expression of ten different herbal preparations. Ten different herbal preparations including three forms of Ginseng (A) White, (B) Red, (C) American, (D) Cistanche tubulosa (Schenk) R. Wight, (E) sinensis sinensis, (F) Ganoderma Lucidium, (S) Scutellaria baicalensis, (P) Georgi Paeonia lactiflora Pall., (Z) Ziziphus jujuba Mill., (G) Glycyrrhiza uralensis Fisch., PHY906-6, 7, 8 and HQT-F were examined. Each preparation was used to treat HepG2 cell cultures for a period of 24 hours at the standard IC50 dose for the herbal or formulation with gene expression levels measured using the Affymetric UA133A chip. Combining data from eleven different herbs or herbal formulations generated a total of 524 genes in the union set that are regulated with greater than a 1.7 fold change compared with a buffer-treated control. This color-coded gene expression map shows the unique expression patterns for these 524 genes observed for different herbal preparations. While high similarity was observed for the three ginseng varieties, there were still subtle differences that distinguished the varieties. Similarly, although three clinical batches PHY906-6, 7 and 8 were nearly identical, there were subtle differences compared with the bioresponse gene expression pattern of HQT-F.