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Figure 2 | Chinese Medicine

Figure 2

From: Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes

Figure 2

Effects of AC on cell proliferation and cell viability in primary human T lymphocytes. Primary human T lymphocytes (2 × 105/well) were stimulated with or without anti-CD3 (1 μg/ml)/CD28 (3 μg/ml) Ab and treated with medium, 0.1%DMSO, or the indicated concentration of AC, or CsA (2.5 μM). (A) After incubated for 72 hours, the proliferation of T lymphocytes was detected by tritiated thymidine uptake (1 μCi/well). After incubated for 16 hours, the cells were harvested by an automatic harvester, then radioactivity was measured by liquid scintillation counting. (B) After 96 hr incubation, T cells were harvested and numbers of total, viable, and nonviable cells were counted after trypan blue staining. Each bar represents the mean ± SD of three independent experiments. ## P < 0.01: vs. the cells treated with DMSO. ** P < 0.01: vs. the cells treated with DMSO and anti-CD3/CD28 Ab.

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