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Figure 4 | Chinese Medicine

Figure 4

From: Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes

Figure 4

Effects of AC on IL-2 and IFN-γ transcripts in primary human T lymphocytes induced by anti-CD3/CD28 Ab. Primary human T lymphocytes (5 × 106) activated with or without anti-CD3 (1 μg/ml)/CD28 (3 μg/ml) Ab in the presence or absence of 6.25, 12.5 or 25 μM AC or CsA (2.5 μM) for 18 hr. The total cellular RNA was isolated from T lymphocytes and aliquots of 1 μg of RNA were reverse-transcribed for synthesis of cDNA. Briefly, 10 μl of cDNA was applied for the PCR test. The PCR was done as described in Materials and Methods. After the reaction, the amplified product was taken out of the tubes and run on 2% agarose gel. (A) and (B): Lane 1 - medium, Lane 2 - 0.1% DMSO, Lanes 3 to 5 - 6.25, 12.5 and 25 μM AC, Lane 6 - anti-CD3/CD28 Ab, Lane 7 - DMSO and anti-CD3/CD28 Ab, Lanes 8 to 10 - 6.25, 12.5 or 25 μM AC and anti-CD3/CD28 Ab. (C) and (D): Lane 1 - 0.1% DMSO, Lane 2 - CsA, Lane 3 - DMSO and anti-CD3/CD28 Ab, Lane 4 - CsA and anti-CD3/CD28 Ab. Graphical representation of laser densitometry of IL-2 and IFN-γ mRNA expression in resting or anti-CD3/CD28 Ab-stimulated PBMC in the presence or absence of AC or CsA. Each band was quantitated using laser-scanning densitometer SLR-2D/1D (Biomed Instruments, USA). The ratio of each cytokine mRNA to GAPDH mRNA was calculated. Each bar is the mean ± SD of three independent experiments. ## P < 0.01: vs. the cells treated with DMSO. ** P < 0.01: vs. the cells treated with DMSO and anti-CD3/CD28 Ab.

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