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Figure 5 | Chinese Medicine

Figure 5

From: Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes

Figure 5

Effects of AC on NF-AT activation. Jurkat cells (5 × 104) were transfected with pGL4.30 (luc2P/NFAT-RE/Hygro) by Lipofectamin™ 2000 (Invitrogen, USA) for 24 hours according to the manufacturer's instructions. Then, the cells were cultured with anti-CD3 (1 μg/ml)/CD28 (3 μg/ml) Ab in the presence or absence of AC (6.25, 12.5 and 25 μM) or CsA (2.5 μM) for four hours. Total cell lysates were extracted with 1× reporter lysis buffer (Promega, USA), then 10 μg of total cell lysates were used to determine luciferase activity by the Luciferase Assay System (Promega, USA). Each bar is the mean ± SD of three independent experiments. ## P < 0.01: vs. the cells treated with DMSO. ** P < 0.01: vs. the cells treated with DMSO and anti-CD3/CD28 Ab.

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