Figure 6

EBE reduced apoptosis induced by 6-OHDA in PC12 cells. Cells were stained with DNA-binding fluorescent dye Hoechst 33342. (A) Control: untreated group; (B) 6-OHDA-treated group (1 mM, 8 hours): chromatin condensation and DNA fragmentation were indicated by the white arrows; (C-F) EBE-pretreated groups (25, 50, 100 and 200 μg/mL respectively, 12 hours), followed by 6-OHDA exposure (1 mM, 8 hours): less apoptotic bodies were identified, colony reduction and cell shrinkage induced by 6-OHDA were also reversed.