Figure 7

EBE inhibited 6-OHDA-induced nitric oxide (NO) over-production in PC12 cells. PC12 cells were pretreated with or without 250 μM L-NAME, 100 μM SNP and 25, 50, 100 and 200 μg/mL EBE for 12 hours, then exposed to1 mM 6-OHDA for another hour. (A-H) Intracellular NO was identified using fluorescent indicator, DAF-FM diacetate; (I) The NO fluorescent intensity was quantified by a multi-label counter. ⁺⁺ P = 0.008 versus control group (without 6-OHDA treatment); * P = 0.044 versus OHDA group; ** P = 0.005 versus OHDA group. All experiments were repeated 3 times.