From: Array-based techniques for fingerprinting medicinal herbs
 | Oligonucleotide microarrays | Gene-probe based microarrays | DArT™ | SDA | SSH-based arrays |
---|---|---|---|---|---|
Sequence information required | Yes | Yes | No | No | No |
Restriction enzymes used | None | None | Yes [usually one rare cutter (Pst I) and one frequent cutter (Taq I/Bst NI/Hae III)] | Yes [two frequent cutters (Hae III and Alu I)] | Yes [one frequent cutter (Rsa I)] |
Subtraction Suppression Hybridisation required | No | No | No | Yes (one) | Yes (multiple pairwise) |
Probe preparation | Chemical synthesis | PCR amplified products | Selective amplified products of the digested DNA fragments | Subtracted DNA fragments | Subtracted/Restriction digested DNA fragments |
Target preparation | PCR amplified products | PCR amplified products | Selective amplified products of the digested DNA fragments | Restriction digested DNA fragments | the other choice against probe preparation |
Dye system used* | Single-dye | Single-dye | Single/Dual-dye | Single-dye | Single/Dual-dye |
Polymorphism rate | Species specific | Species specific | Up to 27% | Up to 68% | Up to 42.4% |