Effect of Fractions I and III of the ALC extract on LPS-stimulated 24 hours macrophage production of NO. Murine macrophages (RAW 264.7 cells) were pre-treated with or without the AQ and ALC extracts (10, 50, 100, 200 μg/ml) for two hours after which LPS (1 μg/ml) was added, and the NO content of the culture supernatants were determined by Griess reaction assay 24 hours later. Three independent experiments were performed and the data were shown as mean ± SD. Datasets were evaluated by ANOVA. * Values P < 0.001 compared to the LPS positive control were statistically significant.