Figure 4
Effects of CTS on object discrimination performance of SAMP8 with and without ischemic insult in the object location test (OLT). The OLT was conducted on days 23-25 after T2VO operation. Each datum represents the mean ± SD (ten mice per group). (A) The data from the sample trials of the OLT. The animal was placed into the arena where two identical sample objects made of glass (objects A1 and A2) were placed in two adjacent corners of the arena and was allowed to explore for five minutes. There was no significant difference in performance in the sample phase trial among the groups. (B) The data from the test phase trials conducted ten minutes after the sample phase trials. In the test phase trials, the time animals spent exploring an object placed in the familiar and a new location was measured during a 5-minute observation period. ***P < 0.001 and **P < 0.01 vs. the time spent exploring the familiar location (paired t-test). (C) Discrimination index (DI) in the OLT. DI was calculated as described in the text. ###P < 0.001 vs. vehicle-treated SAMR1 group (t-test). ***P < 0.001 vs. vehicle-treated sham- or T2VO-SAMP8 groups (two-way ANOVA).