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Table 2 Calibration curves, detection limits and quantification limits of the saponins by HPLC-UV

From: Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS

Peak No. Saponins M.W. Calibration curvea Linear range (μg/ml) R 2 LOD (μg/ml)
21 Ginsenoside Rk3 619 y = 6.7519x - 7.6085    
22 Ginsenoside Rh4 619 y = 6.7519x - 7.6085    
12 Ginsenoside Rh1 637 y = 6.7519x - 7.6085 4.28-68.5 0.9993 2.14
14 Ginsenoside F1 637 y = 6.7519x - 7.6085    
17 Notoginsenoside T5/Unkown 752 y = 5.4845x - 4.8387    
19 Notoginsenoside T5/Unkown 752 y = 5.4845x - 4.8387    
18 Unkown 765 y = 5.4845x - 4.8387    
20 Unkown 765 y = 5.4845x - 4.8387    
26 Ginsenoside Rk1 765 y = 5.4845x - 4.8387    
27 Ginsenoside Rg5 765 y = 5.4845x - 4.8387    
11 Ginsenoside Rg2 783 y = 5.6715x - 5.6679 3.34-53.5 0.9993 1.67
23 20(S)-Rg3 783 y = 5.4845x - 4.8387 2.95-47.3 0.9990 1.48
24 20(R)-Rg3 783 y = 5.0923x - 2.8995 2.63-42.0 0.9994 1.75
25 Ginsenoside F2 783 y = 5.4845x - 4.8387    
2 Ginsenoside Rg1 800 y = 5.1367x - 76.471 16.64-1065 0.9990 10.29
5 Ginsenoside Rf 800 y = 5.1367x - 76.471    
8 SC1 901 y = 4.3254x - 5.0843    
1 Notoginsenoside R1 932 y = 4.3254x - 5.0843 10.26-492.5 0.9997 7.42
3 Ginsenoside Re 945 y = 4.4123x - 29.465 43.28-692.5 0.9993 4.73
15 Ginsenoside Rd 945 y = 4.1199x - 5.5681 16.64-532.5 0.9993 4.43
16 Notoginsenoside K 945 y = 4.1199x - 5.5681    
13 Ginsenoside Rb2 1077 y = 3.8757x + 2.4182 4.84-77.5 0.9995 1.95
7 Notoginsenoside I 1092 y = 3.8757x + 2.4182    
9 Ginsenoside Rb1 1107 y = 3.5815x - 29.548 15.98-1022.5 0.9992 7.91
10 Notoginsenoside Fc 1209 y = 3.5815x - 29.548    
4 Notoginsenoside R4 1240 y = 3.5815x - 29.548    
6 Notoginsenoside Fa 1240 y = 3.5815x - 29.548    
  1. a y: peak area of analyte; x: concentration of analyte (μg/ml)