Lesions developed by sham- or EA-treated mice after L. major infection. BALB/c mice were treated with EA (15/30 Hz) at the Zusanli acupoint (ST36) for 20 min/d for 5 d. After the final EA session, the mice were infected with 2 × 106 stationary-phase promastigotes of L. major into one of the hind footpads. The lesion size was calculated as the difference between the left and right footpad measurements. The sham-treated mice received only needle insertion into a non-acupoint (gluteal muscle) without electrical stimulation. The data are represented as mean ± SD of two independent experiments containing three mice in each group. *P < 0.05, significant difference between sham- and EA-treated mice by two-way ANOVA followed by a Tukey test.