Anxiety behaviour in the EPM test. C57BL/6 J mice (N = 8) completed the EPM test. The time spent (in seconds) in the open arms and closed arms during the 10-min test period was evaluated. After treatment with fluoxetine, XYS, and XYS/RTSES, the ratios of entries onto open arms to closed arms were calculated at 2 weeks (A) and 4 weeks (B), respectively. The numbers of total entries (crossing between open and closed arms) were recorded at 2 weeks (C) and 4 weeks (D). Each value is expressed as the mean ± SD of 8 mice. *P < 0.05, **P < 0.01, ***P < 0.001, significant difference compared with control C57BL/6 J mice. #P < 0.05, ##P < 0.01, ###P < 0.001, significant difference compared with XYS alone.