The effects of puerarin on Akt/GSK-3β/β-catenin signaling pathway. (A-B) Effects of puerarin on β-catenin protein expression in cytoplasm and nuclear in 3T3-L1 cells. The effect of puerarin on β-catenin was determined by western blotting. (C) Effects of puerarin on β-catenin translocation in 3T3-L1 cells. β-catenin expression was labeled with anti-β-catenin antibody and an Alex a fluor 488-conjugated secondary antibody (green). Magnification × 400. (D-E) The protein of AKT1, AKT1 (phosphor-Ser473), GSK-3β and GSK3β (phospho-Ser9) were detected by western blot assay and quantification of immunoblots.