Figure 3

Concentration-dependent induction of apoptosis in THP-1 cells. Cells were cultivated for 24 h in the presence of different concentrations of partially purified P. linteus polysaccharide extract, respectively of ethanol extract of P. linteus. Cells were collected by centrifugation, fixated in 4% buffered formaldehyde and stained by AO/EB. A minimum of 500 cells was counted per point. Observation at excitation/emission at 485/530 nm, using a 20× objective. (a) Dose effect graph. (b) Microphoto of THP-1 cells treated by 375 μg P. linteus polysaccharide extract/mL medium. * and ^#P < 0.05, significantly different from the previous values of the same concentration range.