Figure 5

Confirmation of genes expression identified in microarray by RT-PCR. Limited number of genes identified to be regulated by frankincense, sandalwood essential oils or both from microarray results were confirmed by RT-PCR. (A) Representative images of RT-PCR amplified JUN, DUSP1, DNAJB4, PMAIP1, ZNF311, and β-actin from frankincense and sandalwood essential oils-treated J82 cells were presented. (B) Relative changes of JUN, DUSP1, PMAIP1, and ZNF311 transcripts expression were determined by quantitative RT-PCR analysis. Data were presented as mean (SD) from at least 3 independent experiments.