Figure 1

Effects of phytochemicals on (A) the autonomous Gal4-hLXRα LBD transactivity and (B) the LXRα agonist-stimulated transcript levels of SREBP-1c. (A) Gal4-LXRα LBD transactivity was measured in the presence of 1 μM T0901317 and 10 μg/mL natural compounds by a Gal4-TK- luciferase assay. Data were presented as means (SD) from three independent experiments with duplicate determinations. (B) Effects of natural compounds on T0901317-stimulated SREBP-1c mRNA levels in primary hepatocytes. mRNA levels were measured by conventional RT-PCR. Quantitation of band intensities was performed using ImageJ (NIH, Bethesda, MD, USA). Data are presented as means (SD) from three independent experiments. Statistical analysis was performed using one-way ANOVA. P* = P value for Bonferroni correction. T1317, T0901317; War, warangalone 4′-methyl ether; LicA, licochalcone A; SigK, sigmoidin K; Alb, albaspidin P-P; Cur, curcumin; Vin, ɛ-viniferin; Mac, macelignan.