Fig. 6

PNFS markedly suppressed the activation of LPS-stimulated MAPK signaling pathway in RAW264.7 macrophages. a PNFS suppressed the P38 activation in LPS-stimulated RAW264.7 macrophages. b PNFS suppressed the ERK1/2 activation in LPS-stimulated RAW264.7 macrophages. c PNFS suppressed the JNK activation in LPS-stimulated RAW264.7 macrophages. Data of the production of P-P38, total P38, P-ERK1/2, total ERK1/2, P-JNK and total JNK were all expressed as the mean (SD) of 3 independent experiments. One-Way ANOVA test was used to analyzed the data and the results were F = 45.104; P < 0.001, F = 0.322; P = 0.736, F = 7.330; P = 0.024, F = 1.019; P = 0.416, F = 11.180; P = 0.009 and F = 2.144; P = 0.198, respectively. Then, data of the production of P-P38, total P38, P-ERK1/2, total ERK1/2, P-JNK and total JNK were all counted by SNK and LSD multiple comparisons to determine the statistical difference between two groups. The P values represented the statistical differences between each group and the corresponding positive control (without PNFS and with LPS treated). * means P < 0.05, ** means P < 0.01 and *** means P < 0.001