Fig. 6

Inhibition of the PI3K-Akt-mTOR signaling pathway in CNE-1 cells by CME. After treatment with the indicated concentrations of CME for 24 h, cell lysates were subjected to western blot analysis using the indicated antibodies against PI3K p110α, Akt and p-Akt (Thr³⁰⁸ and Ser⁴⁷³), mTOR and p-mTOR (Ser²⁴⁴⁸ and Ser²⁴⁸¹). β-actin served as a loading control