Fig. 4

Effects of QDP on macrophage infiltration in colons of DSS-treated mice. A Immunohistochemical (IHC) analysis of colonic macrophages by F4/80 marker [a–f representative images (a control; b DSS model; c SASP; d 0.77 g/kg QDP; e 1.54 g/kg QDP; and f 3.08 g/kg QDP), g number of macrophages]; B flow cytometric analysis of macrophages in the colon lamina propria (LP) (a control; b DSS model; c 1.54 g/kg QDP; and d percentage of macrophages in total LP cells). Colitis was induced in all groups except control group. QDP and SASP were administered to mice from day 6 to 12. On day 13, mice were sacrificed. The colon section was evaluated by IHC analysis with F4/80 marker (n = 7–9). For flow cytometric analysis, the population of macrophage in lamina propria mononuclear cells from whole colonic tissue was determined by CD11b and F4/80 markers (n = 5 for normal control group, and n = 6 for DSS-treated groups). Data were expressed as mean ± SD