Fig. 3

Effects of the tendon extract on cell viability (a) and differentiation in RANKL induced-Raw264.7 cells (b). a Raw 264.7 cells were cultured with different concentration of tendon extracts for 4 days. Cell viability was determined by MTT assay. Results were obtained from three independent experiments (4–6 replicate for each experiment) and expressed as mean ± SD. **P ≤ 0.01, ***P ≤ 0.001 versus the control (“0”). b Raw 264.7 cells were cultured with basal medium (Neg), 50 ng/ml RANKL (Vehicle) and 100 µg/mL of each tendon extract for 4 days. A representative photo for TRAP staining (upper, magnification: ×40) and the quantitative comparison (lower) are shown. Results were obtained from three independent experiments (2–3 replicates for each experiment) and expressed as mean ± SD. ***P ≤ 0.001 versus the vehicle control group