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Table 2 Relative gene expression in Raw 264.7 (A) and UMR106 cells (B)

From: Differentiation of deer tendons from cattle tendons by a loop-mediated isothermal amplification (LAMP) test and bone remodeling bioassays

(A) NFATc TRAP CTSK
Vehicle control 1 1 1
Cattle tendon (100 µg/mL) 0.45 ± 0.17** 0.34 ± 0.11** 0.40 ± 0.18*
Deer tendon (100 µg/mL) 0.74 ± 0.14 0.55 ± 0.20* 0.72 ± 0.18
(B) OPG RANKL OPG/RANKL
Vehicle control 1 1 1
Cattle tendon (200 µg/mL) 1.28 ± 0.82 0.90 ± 0.54 1.37 ± 0.16
Cattle tendon (400 µg/mL) 1.82 ± 0.50 1.18 ± 0.27 1.58 ± 0.49
Deer tendon (200 µg/mL) 1.40 ± 0.76 0.88 ± 0.25 1.56 ± 0.66
Deer tendon (400 µg/mL) 1.72 ± 0.86 0.82 ± 0.20 2.09 ± 0.96*
  1. (A) Raw 264.7 cells were treated with RANKL and 100 µg/mL of tendon extracts for 72 h. (B) UMR 106 cells were treated with differentiation medium together with 200 or 400 µg/mL of both tendon extract for 72 h. For both A and B, the expression of respective genes was determined by real-time PCR and normalized with the housekeeping gene GAPDH. Results were obtained from 4 to 5 independent experiments in duplicate and expressed as mean ± SD
  2. P ≤ 0.05; ** P ≤ 0.01 versus vehicle control