Differentiation of deer tendons from cattle tendons by a loop-mediated isothermal amplification (LAMP) test and bone remodeling bioassays

Table 2 Relative gene expression in Raw 264.7 (A) and UMR106 cells (B)

(A)	NFATc	TRAP	CTSK
Vehicle control	1	1	1
Cattle tendon (100 µg/mL)	0.45 ± 0.17**	0.34 ± 0.11**	0.40 ± 0.18*
Deer tendon (100 µg/mL)	0.74 ± 0.14	0.55 ± 0.20*	0.72 ± 0.18

(B)	OPG	RANKL	OPG/RANKL
Vehicle control	1	1	1
Cattle tendon (200 µg/mL)	1.28 ± 0.82	0.90 ± 0.54	1.37 ± 0.16
Cattle tendon (400 µg/mL)	1.82 ± 0.50	1.18 ± 0.27	1.58 ± 0.49
Deer tendon (200 µg/mL)	1.40 ± 0.76	0.88 ± 0.25	1.56 ± 0.66
Deer tendon (400 µg/mL)	1.72 ± 0.86	0.82 ± 0.20	2.09 ± 0.96*

(A) Raw 264.7 cells were treated with RANKL and 100 µg/mL of tendon extracts for 72 h. (B) UMR 106 cells were treated with differentiation medium together with 200 or 400 µg/mL of both tendon extract for 72 h. For both A and B, the expression of respective genes was determined by real-time PCR and normalized with the housekeeping gene GAPDH. Results were obtained from 4 to 5 independent experiments in duplicate and expressed as mean ± SD
* P ≤ 0.05; ** P ≤ 0.01 versus vehicle control

ISSN: 1749-8546