Table 1 Primer sequences for RT-PCR
From: Actions of Huangqi decoction against rat liver fibrosis: a gene expression profiling analysis
Gene | Description | Primer sequences | |
|---|---|---|---|
Forward | Reverse | ||
PDGFra | Platelet derived growth factor receptor, alpha polypeptide | GAAGGTGGTTGAAGGAACAGC | AGGCTCCCAGCAAGTTTACAA |
PDGFrb | Platelet derived growth factor receptor, beta polypeptide | CGCGTGCGTCTGTTTTCAATTT | GCGTGGGCTGTGGAATTTCTAA |
PDGFb | Platelet derived growth factor beta polypeptide | GGCCTTCTTAAAGATTGGCTTCT | GCCTCATAGACCGCACCAAC |
PDGFd | platelet derived growth factor D | CCCATTCGGAGGAAGAGAAG | ATCAGGAAGTTGGCGGACG |
COL1A1 | Collagen, type I, alpha 1 | ACGTCCTGGTGAAGTTGGTC | ACCAGGGAAGCCTCTCTCTC |
COL1A2 | Collagen, type I, alpha 2 | GGAGGGAACGGTCCACGAT | GAGTCCGCGTATCCACAA |
COL5A2 | Collagen, type V, alpha 2 | GGAAATGTGGGCAAGACTGT | TTGATGGTGGTGCTCATTGT |
THBS1 | Thrombospondin 1 | AACGTGGATCAGAGGGACAC | GTCATCGTCATGGTCACAGG |
ITGA5 | Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) | GTCGGGGGCTTCAACTTAGAC | CCTGGCTGGCTGGTATTAGC |
IL1R1 | Interleukin 1 receptor, type I | AGAGGAAAACAAACCCACAAGG | CTGGCCGGTGACATTACAGAT |
β-actin | Actin, beta | TCCTGTGGCATCCACGAAACT | GAAGCATTTGCGGTGGACGAT |