Skip to main content
Fig. 4 | Chinese Medicine

Fig. 4

From: Novel cycloartane triterpenoid from Cimicifuga foetida (Sheng ma) induces mitochondrial apoptosis via inhibiting Raf/MEK/ERK pathway and Akt phosphorylation in human breast carcinoma MCF-7 cells

Fig. 4

Cell-cycle arrest induced by ADHC-AXpn in MCF-7 cells. a Cell cycle profiles of MCF-7 cells after treatment with ADHC-AXpn for 24 or 48 h. Cells exposed to indicated concentrations of ADHC-AXpn were collected, fixed with 75 % ethanol overnight, and stained with PI for 30 min; cell-cycle profiles were then determined by flow cytometry. Doxorubicin (dox) exposure at 5 μM was used as a positive control. b Changes in cell-cycle regulator protein expression after exposure to ADHC-AXpn in MCF-7 cells. After treatment with ADHC-AXpn, total cell lysates were immunoblotted to detect CDK1, p-CDK1 (Thr161) and cyclin B1 expression levels, using specific antibodies. β-actin served as a loading control

Back to article page

Chinese Medicine

ISSN: 1749-8546

Contact us