Table 2 Descriptions of the 14 candidate reference genes and their primer sequences for qRT-PCR
Gene | Gene description | Primer sequences (forward/reverse) | Amplicon length (bp) | Access number | Total copy numbers |
|---|---|---|---|---|---|
GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | TGTTCGTCTGCGGTGTCA/AGTGGACGGTGGTCATCAG | 150 | KJ716556 | 1 |
MAPK | Mitogen-activated protein kinase | CACATCCAGCACGAGAACAT/GGAGGATCTGGTAGAGGAAGTA | 163 | KJ716546 | 10 |
β-Act | Beta actin | ATGCGAGGTTATGCGTTCA/CCGACCATCTGGGAGTGTAT | 156 | KJ716554 | 2 |
RPB2 | RNA polymerase subunit 2 | ACCAACTTCCTCGTCAGAATG/TCCTGATTGTATCCGCTGTAAC | 161 | KJ716552 | 1 |
RPB1-1 | RNA polymerase subunit 1 | GGCTTACAACAGGTCGTCAA/CGTGGCGTCCTCAATAACTT | 153 | KJ716547 | 2 |
RPB1-2 | RNA polymerase subunit 1 | AGGATGACGAAGCAGAGGAA/TGGCATTGGGCAGGTTCT | 168 | KJ716548 | |
his3-1 | Histone 3 | AGTCCACGGAACTCCTAATCA/AGCGGCTAAGTTGGTGTCT | 167 | KJ716557 | 3 |
his3-2 | Histone 3 | CGACGGAGTTGCTCATCAG/GTGGATCGCAGCCAGATTC | 170 | KJ716544 | |
APT | Adenine phosphoribosyltransferase | ACCTGAGGAGTCTGCTGAAG/TTGTGGAATAGTGTGCGATGT | 149 | KJ716549 | 1 |
SAMDC | S-adenosylmethionine decarboxylase | GCTTCTACTCTCGCAAGGC/GATATACAGCAGCCAGTGGTC | 155 | KJ716550 | 1 |
RP | Ribosomal protein | TGTCGCTCTCCTCAAGTCC/CGGAATGCCTTGACGATACC | 165 | KJ716551 | 1 |
β-Tub | Beta tubulin | GCCAACATACGGTGATCTGAA/GAAGAAGTGAAGACGAGGGAAT | 142 | KJ716555 | 1 |
EIF | Eukaryotic translation initiation factor | TGACGATGACAGCGATGAAG/CACCTGGACTGCCTTATGC | 145 | KJ716545 | 1 |
CYP | Cyclophilin | CATGGCTTCGGCTACAAGG/TTGGTGTGCTTGAGCTTGAA | 152 | KJ716553 | 3 |