Chi-Ju-Di-Huang-Wan protects rats against retinal ischemia by downregulating matrix metalloproteinase-9 and inhibiting p38 mitogen-activated protein kinase

Table 4 Terminal deoxynucleotidyl-transferase dUTP nick end-labeling (TUNEL)

Group	1	2	3	4
Apoptotic cell No. on day 1	0	1.40 ± 0.55^**	0.80 ± 0.45	0.40 ± 0.55^†
Apoptotic cell No. on day 7	0	1.40 ± 0.55^**	0.80 ± 0.84	0.60 ± 0.55^†

No TUNEL-positive apoptotic cell was observed 1 or 7 days after the sham procedure (Sham; Group 1). After retinal ischemia plus 1 or 7 days of reperfusion and pretreatment with vehicle (I/R + vehicle; Group 2), the number of apoptotic cells was significantly (** P < 0.01) increased in the retinal ganglion cell layer. In contrast, this significant increase was dose-dependently (with a less effect at 2.8 g/kg/day, I/R + CJDHW2.8; Group 3) and significantly (^† P < 0.05; at 4.2 g/kg/day, I/R + CJDHW4.2; Group 4) inhibited by pretreatment with CJDHW. The results are the mean ± SD (n = 5)

ISSN: 1749-8546