Table 2 Oligonucleotide primers used for molecular identification
From: An investigation of fungal contamination on the surface of medicinal herbs in China
Primer name | Primer sequence | Amplification product (bp) | Annotation | Gene targeted |
|---|---|---|---|---|
Wen1-F | 5′–TCCAACCTCCCACCCGTGTTTA–3′ | 400 | This study | ITS1-5.8S-ITS2 |
Wen1-R | 5′–AAGCCCCTACGCTCGAGGA–3′ | |||
ITS1 | 5′–TCCGTAGGTGAACCTGCG–3′ | 500 ~ 700 | [32] | |
ITS4 | 5′–TCCTCCGCTTATTGATATGC–3′ |