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Table 4 Comparison result by four methods (Psoraleae)

From: A simple method for HPLC retention time prediction: linear calibration using two reference substances

Method Maximum of ΔtR/min Average of ΔtR/min Number of positive columnsa
Unadjusted RR 3.494 0.804 2
Adjusted RR 3.001 0.886 3
Prediction by two points 2.194 0.599 5
Validation by multiple points 1.689 0.465 9
  1. aThe columns which meet the following requirements are called positive column, (1) the resolution of peaks meets the requirements; (2) ΔtR of all pending test compounds are no more than tRL (for Psoraleae is 1.2 min)