Fig. 2

Siegesbeckia pubescens Makino (SP) or SP with Pam3CSK4-stimulated did not significantly affect the cell viability and cytotoxicity (n = 3). RAW 264.7 cells were treated with SP with different concentrations for 24 h. a The cell viability was measured by MTT assay and c cell cytotoxicity was measured by LDH assay. RAW 264.7 cells were pretreated with SP for 4 h before Pam3CSK4 (200 ng/mL) stimulation for another 12 h. b The cell viability was measured by MTT assay and d cell cytotoxicity was measured by LDH assay