Fig. 6
Effects of Siegesbeckia pubescens Makino (SP) on NF-κB pathways (n = 3). RAW 264.7 cells were pretreated with SP (0, 50, 100 and 200 μg/mL) for 2 h and followed with Pam3CSK4 (200 ng/mL) addition for 4 h. CU-CPT22 (CU) is selected as the positive control. a Proteins was evaluated by Western blotting assay. b, c Quantification was detected by densitometric analysis. d RAW 264.7 cells were transfected with NFκB-luc for 48 h. Cells were pretreated with SP 2 h before Pam3CSK4 (200 ng/mL) stimulation for another 4 h. Luciferase activity was determined by Dual-Glo Luciferase Assay. *P < 0.05 vs. Pam3CSK4-induced and **P < 0.01 vs. Pam3CSK4-induced