Fig. 8

DOPA-1 treatment induces dose-dependent HepG-2 cell apoptosis. A Flow cytometric analysis of HepG-2 cell apoptosis with Annexin V-FITC and PI labeling. Subpanels: (a) untreated control group, (b) 50 μg/mL 5-FU, (c) 50 μg/mL DOPA-1, (d) 100 μg/mL DOPA-1, (e) 200 μg/mL DOPA-1, (f) 400 μg/mL DOPA-1, and (g) the calculated apoptotic rate for each treatment group. B Western blotting analysis and quantitation of Bax and Bcl-2 expression. Data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ^#p < 0.05, ^##p < 0.01, compared to the blank control group