Table 1 Reagent addition to ampoule of each channel (mL)
Channel no | Mitochondria suspension/mL | Buffer B, solution/mL | Sample, solution/mL |
|---|---|---|---|
Ch 1 | 0 | 10 | 0 |
Ch 2 | 6 | 4 | 0 |
Ch 3 | 6 | 3.95 | 0.05 |
Ch 4 | 6 | 3.9 | 0.1 |
Ch 5 | 6 | 3.8 | 0.2 |
Ch 6 | 6 | 3.6 | 0.4 |
Ch 7 | 6 | 3 | 1 |
Channel no | Mitochondria suspension/mL | Buffer B, solution/mL | Sample, solution/mL |
|---|---|---|---|
Ch 1 | 0 | 10 | 0 |
Ch 2 | 6 | 4 | 0 |
Ch 3 | 6 | 3.95 | 0.05 |
Ch 4 | 6 | 3.9 | 0.1 |
Ch 5 | 6 | 3.8 | 0.2 |
Ch 6 | 6 | 3.6 | 0.4 |
Ch 7 | 6 | 3 | 1 |