Fig. 7

Effect of SKN, PTX, or their combinations on expression and activity of PKM2. a A2780/PTX cells were treated with SKN (1 and 2 µM), PTX (1 µM), or combination of SKN/PTX for 2.5 h with or without pretreatment of NAC (5 mM), and then harvested for western blot analysis. Anti-GAPDH was used as an internal standard. Intensity of protein bands was quantified using ImageJ 1.47v. b A2780/PTX cells were treated with SKN (1 µM), PTX (1 µM), or combination of SKN/PTX for 2.5 h with or without pretreatment of NAC (5 mM) and then lysed for Pyruvate Kinase Assay Kit