Fig. 8

RSM induces apoptosis and cell cycle G2/M arrest in MHCC97-H and HepG2 cells. a Flow cytometric analysis of Annexin V/PI double staining in MHCC97-H and HepG2 cells treated with various concentrations of RSM for 24 h. b MHCC97-H and HepG2 cells were treated with RSM at the indicated concentrations for 24 h and subjected to western blotting with antibodies against Bcl-2 and Bax. c Cell cycle analysis revealed that RSM arrested cells at the G2/M checkpoint. MHCC97-H and HepG2 cells were incubated with the indicated dose of RSM for 24 h, stained with Propidium iodide (PI), and DNA content was analyzed via flow cytometry. All data are presented as mean ± SD (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001 versus the control group