Fig. 6

Effects of Sigesbeckia glabrescens Makino (SG) on the relevant pathways. RAW 264.7 cells were pretreated with SG (0, 25, 50, 100 or 200 μg/mL) for 4 h followed by the addition of lipopolysaccharides (LPS, 1 μg/mL) for 12 h. The NF-κB pathway was investigated by a, b Western blotting (p-p65, Ser536) and c a dual-luciferase reporter assay. Indomethacin (IND) was selected as a positive drug. *P < 0.05 vs. LPS-induced cells, **P < 0.01 vs. LPS-induced cells, and ***P < 0.001 vs. LPS-induced cells