Fig. 5
From: Anti-tumor effects of the ethanolic extract of Trichosanthes kirilowii seeds in colorectal cancer
Effects of TKSE on the malignant properties of HT-29 cells. a The expressions of HIF-1α and its downstream gene VEGF were assessed by western blotting in hypoxia-induced HT-29 cells treated with TKSE. b Production of VEGF in hypoxia-induced HT-29 cells treated with TKSE. Cells were treated with various concentrations of TKSE under hypoxia for 24 h. Results are presented as the means ± SDs of triplicate wells. Significance vs. CoCl2-treated group, **p < 0.01, ***p < 0.001. c A wound healing migration assay was used to observe the effects of TKSE on HT-29 cell motility. d Representative images of Matrigel transwell invasion by TKSE-treated HT-29 cells. Original magnification, ×100