Fig. 4

The flavonoids activate IRS1/PI3K/AKT signaling in IR model of 3T3-L1 adipocytes. a Immunofluorescent staining of p-IRS1 in3T3-L1 dipocytes (100 ×). b Immunofluorescent staining of p-PI3K in 3T3-L1 dipocytes (×100). c Immunofluorescent staining of p-Akt in 3T3-L1 dipocytes (×100). d Related to a–c, the quantitative of immunofluorescent staining of p-IRS1, p-PI3K and p-Akt in 3T3-L1 dipocytes. n = 6. ^##p < 0.01, compared with control group; **p < 0.01, compared with model group. e The representative result of western blot analysis of p-IRS1, IRS1, p-PI3K, PI3K, p-Akt and Akt in 3T3-L1 dipocytes. f Related to e, the quantitative of p-IRS1, IRS1, p-PI3K, PI3K, p-Akt and Akt protein expressions in 3T3-L1 dipocytes. n = 6. ^##p < 0.01, compared with control group; **p < 0.01, compared with model group