Fig. 6

PPAR-γ signal is involved in the inhibition effects of EOFAZ on RMCs gliosis induced by HG. PPAR-γ siRNA (a–f) and PPAR-γ plasmid (g–l) were used alone or combined with EOFAZ in RMCs. PPAR-γ silencing efficiency of siRNAs (a, b). Study on PPAR-γ overexpression plasmid efficacy (g, h) and Western blot analysis was used to determine the protein expression of GFAP and VEGF (c, d, i, j. Western blot analysis was used to determine the protein expression of GFAP and VEGF (e, f, k, l). Data are expressed as mean ± SEM. n = 3. ^@P < 0.05, ^@@P < 0.01 vs. siRNA negative group or overexpression vector. *P < 0.05, **P < 0.01 vs. control group, ^#P < 0.05, ^##P < 0.01 vs. HG group, ^$P < 0.05 vs.HG + EOFAZ group