Fig. 5

Western blot analysis of apoptotic proteins. a Effect of SK (3 μM) and 4-OHT (15 μM) alone or in combination on the expression of mitochondria-dependent apoptosis related proteins of MCF-7 cells. b Western blot result of proteins related to PI3K/AKT/Caspase 9 pathway of MCF-7 treated with SK (3 μM) and 4-OHT (15 μM) alone or in combination. c Western blot result of proteins related to cell death receptor pathway of MCF-7 treated with SK (3 μM) and 4-OHT (15 μM) alone or in combination. d Western blot result of ERα expression of MCF-7 treated with SK (3 μM) and 4-OHT (17.5 μM) alone or in combination. e Effect of SK (3 μM) and 4-OHT (17.5 μM) alone or in combination on the expression of mitochondria-dependent apoptosis related proteins of MDA-MB-435S cells. f Western blot result of proteins related to PI3K/AKT/Caspase 9 pathway of MDA-MB-435S treated with SK (3 μM) and 4-OHT (17.5 μM) alone or in combination. g Western blot result of proteins related to cell death receptor pathway of MCF-7 treated with SK (3 μM) and 4-OHT (17.5 μM) alone or in combination separated by SDS-PAGE. Relative band intensity was determined by Image J software. GAPDH served as a loading control. Images are representative of three independent experiments. Data are mean ± S.E.M. from three independent experiments (*P < 0.05, **P < 0.01 compared with the negative control; ^#P < 0.05, ^##P < 0.01 compared with the combination group)