Fig. 3

Influence of OMs on the ΔΨm, ROS production and ATP content. Cells were incubated with OMs at indicated concentrations for 48 h, and then induced with IAPP (250 nM) for 24 h. After treatment cells were washed and incubated with JC-1 for ΔΨm determination (a); or washed and incubated with 2 μM DCFH2-DA for ROS production assay (b); or washed and the ATP contents were determined by a luminescence assay kit based on the luciferase-catalyzed oxidation of d-luciferin (c). Values are mean ± S.E.M of the results from at least three independent experiments. ^##P < 0.01 versus control; *P < 0.05, **P < 0.01 versus the IAPP model