Fig. 8

Effect of porcupine dates extracts and tannic acids on DENV-2 attachment in Vero cells. The extracts or tannic acids were incubated with Vero cells at MNTC together with 30–50 FFU of DENV-2 for 1 h at 4 °C. Heparin was assayed at 800 µg/mL as a control. The mixture containing unadsorbed virus was replaced with overlay medium after washing. After 4 days of incubation, the cells were subjected to immunostaining against DENV. Data were statistically analysed using ordinary one-way ANOVA followed by Dunnett’s and Sidak’s multiple comparison tests. Values represent the mean ± SEM of two independent experiments performed in duplicate. *p < 0.05, ****p = 0.0001, “n.s.” indicates not significant