Table 1 Characterization of ZYD prototype components in vivo by HPLC-ESI-Q-TOF-MS/MS
Peak No. | tR(min) | Precursor ions (m/z) | Formula | Error (mDa) | Fragment ions | Identification | P | U | F | B | Refs. | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Experimental | Theoretical | |||||||||||
P1 | 10.332 | 407.1282* | 407.1195 | C17H22O10 | − 8.70 | 361.1038, 199.0607, 181.0507, 169.0505 | Catalpol | + | + | + | − | [10] |
P2 | 20.658 | 391.1284* | 391.1246 | C15H22O19 | − 3.81 | 211.1011, 183.0662, 165.0543, 139.0410 | Aucubin | + | + | + | − | [10] |
P4 | 23.285 | 731.2325* | 731.2251 | C27H42O20 | − 7.35 | 685.2276, 505.1599, 341.1118, 179.0561 | Rehmannioside D | − | + | + | − | [10] |
P5 | 25.065 | 409.1384* | 409.1351 | C15H24O10 | − 3.05 | 201.0771, 183.0663, 165.0556, 157.0504 | Harpagide | + | + | + | + | [10] |
P6 | 26.149 | 393.1443* | 393.1402 | C15H24O9 | − 4.06 | 185.0560, 179.0526, 167.0716, 113.0238 | Leonuride | + | + | + | + | [10] |
P8 | 29.733 | 373.1216 | 373.1140 | C16H22O10 | − 7.58 | 331.0989, 221.0893, 167.0426, 149.0622 | Geniposidic acid | − | + | + | − | [18] |
P9 | 30.113 | 421.1332* | 421.1351 | C16H24O10 | 1.95 | 213.0732, 195.0662, 183.0657, 169.0437 | 6-O-methylcatalpol | + | + | + | + | [19] |
P10 | 30.783 | 461.1698 | 461.1664 | C20H30O12 | − 3.35 | 461.1698 | Decaffeoylacteoside | − | − | + | − | [10] |
P11 | 31.790 | 375.1317 | 375.1297 | C16H24O10 | 2.03 | 375.1317 | 8-epilogonic acid | − | + | + | − | [9] |
P12 | 36.172 | 487.1527 | 487.1457 | C21H28O13 | − 6.99 | 487.1527 | Cistanoside F | − | − | + | − | [10] |
P14 | 41.765 | 313.0938 | 313.0929 | C14H18O8 | − 0.91 | 313.0938, 229.1749, 137.0605, 123.0439 | Rhamnopyranosyl vanilloyl | − | + | − | − | [20] |
P15 | 42.066 | 475.1845 | 475.1821 | C21H32O12 | − 2.4 | 475.1845 | Darendoside B | − | − | + | − | [10] |
P16 | 43.203 | 607.2290 | 607.2244 | C26H40O16 | − 4.64 | 607.2298, 475.1859, 461.1697, 443.1586, 149.0457, 131.0351 | β-(3-hydroxy-4-methoxyhenyl) ethyl-O-α-L-arabinopyranosyl-(1 → 6)-O-[6-α-L-rhamnpyranosyl-(1 → 3)-β-D-glucopyranoside | − | + | + | − | [10] |
P20 | 49.444 | 183.1028 | 183.1027 | C10H16O3 | − 0.13 | 183.1036, 139.1123, 123.0792 | Rehmapicrogenin | + | + | + | + | [10] |
P23 | 54.309 | 435.2269* | 435.2236 | C19H34O8 | − 3.33 | 389.2370, 179.0539, 161.0451, 119.0348 | Rehmaionoside A/B | − | + | + | − | [10] |
P26 | 56.018 | 799.2778 | 799.2666 | C36H48O20 | − 11.18 | 799.2778 | Jionoside A1/A2 | − | + | + | − | [21] |
P24 | 56.295 | 163.0399 | 163.0401 | C9H8O3 | 0.17 | 145.0270, 119.0499 | p-coumaric acid | + | + | + | − | [10] |
P29 | 60.152 | 193.0511 | 193.0500 | C10H10O4 | − 0.47 | 178.0269, 149.0598, 134.0369, 121,0282 | Ferulic acid | − | + | + | − | [10] |
P31 | 61.894 | 623.2049 | 623.1981 | C29H36O15 | − 6.76 | 461.1676, 315.1084, 161.0241, 135.0445 | Acteoside | − | − | + | − | [10] |
P34 | 63.221 | 769.2634 | 769.2561 | C35H46O19 | − 7.35 | 769.2634 | Scrophuloside B1/B2 | − | − | + | − | [22] |
P36 | 63.473 | 623.2021 | 623.1981 | C29H36O15 | − 3.96 | 623.2021 | Isoacteoside or Forsythoside A | − | − | + | − | [10] |
P37 | 65.538 | 525.1665 | 525.1614 | C24H30O13 | − 5.14 | 525.1665 | 8-O-caffeoyl harpagide | − | − | + | − | [19] |
P39 | 67.938 | 429.2171 | 429.2130 | C21H34O9 | − 4.09 | 249.1512, 231.1370, 187.1472 | Jiocarotenoside A1/A2 | − | + | + | − | [23] |
P41 | 69.475 | 783.2798 | 783.2717 | C36H48O19 | − 8.10 | 607.2294, 589.2154, 461.1666, 193.0500 | Angoroside C | + | − | + | − | [10] |
P42 | 70.273 | 783.2840 | 783.2717 | C36H48O19 | − 12.30 | 783.2840, 829.4135 | Isoangoroside C | + | − | + | − | [10] |
P47 | 70.428 | 637.2199 | 637.2138 | C30H38O15 | − 6.11 | 461.1706, 193.0512, 149.0577, 134.0372 | Leucosceptoside A | − | − | + | − | [21] |
P52 | 75.561 | 651.2397 | 651.2294 | C31H40O15 | − 10.26 | 651.2397 | Cistanoside D | − | − | + | − | [24] |
P55 | 79.343 | 539.1823* | 539.1770 | C24H30O11 | − 5.29 | 493.1715, 345.1210, 183.0662, 165.0556 | Harpagoside | + | − | + | + | [10] |
P56 | 81.455 | 147.0450 | 147.0452 | C9H8O2 | 0.15 | 147.0450 | Cinnamic acid | + | − | + | − | [10] |
P62 | 98.429 | 345.1014 | 345.0980 | C18H18O7 | − 3.42 | 345.1014 | 5,7,2′,4′-tetradihydroxy-8-methoyl-6-methyl-homoisoflavanone | − | − | + | − | [10] |
P74 | 104.020 | 359.1161 | 359.1136 | C19H20O7 | − 2.47 | 359.1161 | Ophiopogonanone E | − | + | + | − | [10] |
P75 | 105.884 | 343.1215 | 343.1187 | C19H20O6 | − 2.79 | 343.1215 | 5-7-4′-trihydroxy-5′-methoxy-6,8-dimethyl hamoisoflavanone | − | − | + | − | [25] |
P77 | 107.848 | 327.0905 | 327.0874 | C18H16O6 | − 3.09 | 327.0905 | Ophiopogonone A | − | − | + | − | [10] |
P78 | 110.065 | 339.0898 | 339.0874 | C19H16O6 | − 2.39 | 339.0898 | Methylophiopogone A | − | + | + | − | [10] |
P79 | 110.853 | 341.1059 | 341.1031 | C19H18O6 | − 2.84 | 206.0587, 178.0258 | Methylophiopogonanone A | − | − | + | − | [10] |
P80 | 111.626 | 327.1260 | 327.1238 | C19H20O5 | − 2.20 | 327.1260 | Methylophiopogone B | − | − | + | − | [10] |