Fig. 3
DHA inhibited cell proliferation in Hep-2 cells. a CCK8 was used to test the inhibitory effect of DHA on the proliferation of Hep-2 cells. Hep-2 cells were treated with DHA as indicated (mean ± SD, n= 3). b The IC50 values of cells were measured at 24, 36, and 48 h. IC50 values were obtained from the cytotoxicity curves using the SOFTmax PRO software. c Representative photographs of clonogenic assay. Hep-2 cells were treated with 20.5 μM DHA, 40 μM Etoposide, and DMSO (NC group) for 24 h, respectively. All experiments were performed for at least three times. A representative result was presented. Statistical analysis was conducted on the clone number after 14-day incubation. The inhibitory effects of growth and proliferation were calculated by number of formed cell clones. Data are shown as mean ± SD (n = 3). *P < 0.05 versus NC group