An effective UFLC–MS/MS method used to study pharmacokinetics of major constituents of Fukeqianjin formula in rat plasma

Table 5 The recovery and matrix effect of 19 compounds in FKQJF

Analyte	Spiked (ng/mL)	Recovery		Matrix effect
Analyte	Spiked (ng/mL)	Mean	RSD %	Mean	RSD %
1	500	91.34	8.16	83.40	4.46
	250	117.65	10.34	86.26	13.09
	50	95.57	9.65	95.83	12.51
2	500	82.25	6.95	95.64	14.43
	250	88.18	5.16	118.82	9.91
	50	95.72	13.70	83.95	13.26
3	500	112.88	4.17	86.16	10.51
	250	81.64	9.85	98.74	14.77
	50	92.36	14.37	94.51	12.37
4	500	96.00	10.04	109.41	10.74
	250	112.34	5.75	96.07	7.33
	50	93.48	12.31	81.89	7.77
5	600	116.92	7.90	99.37	11.80
	300	93.25	7.38	81.76	7.84
	60	113.16	11.01	90.40	14.03
6	600	116.07	14.09	95.91	5.33
	300	90.96	9.27	101.58	7.86
	60	113.50	10.86	83.42	4.92
7	500	94.80	10.92	83.83	4.18
	250	84.24	13.75	80.63	11.34
	50	86.90	14.70	105.95	14.40
8	500	114.26	10.58	106.24	11.57
	250	97.53	7.22	91.89	8.13
	50	82.58	8.09	100.86	4.65
9	150	88.02	5.08	115.13	5.53
	75	84.66	4.90	114.37	9.09
	25	97.35	5.71	96.57	11.85
10	500	114.19	12.24	112.83	4.96
	250	119.00	13.92	116.16	13.93
	50	93.86	11.51	88.16	4.67
11	200	103.16	5.57	88.83	6.67
	100	100.81	5.27	102.26	6.90
	10	90.40	12.70	80.62	11.88
12	600	113.68	8.76	114.11	8.79
	300	94.96	14.89	85.75	7.80
	60	111.70	4.41	119.18	11.76
13	200	94.08	14.89	119.79	12.86
	100	114.75	9.32	80.43	4.68
	10	85.57	11.82	85.26	6.47
14	500	119.18	6.16	83.89	6.95
	250	112.28	7.14	84.01	9.14
	50	119.90	10.03	114.12	7.35
15	600	82.03	6.08	91.20	8.50
	300	80.71	5.05	106.55	11.58
	60	82.33	7.78	96.12	12.48
16	500	82.87	9.34	113.66	4.04
	250	96.22	9.00	100.13	14.14
	50	101.27	11.48	88.82	14.28
17	600	91.18	11.92	89.61	9.44
	300	90.15	10.63	99.43	10.45
	60	101.00	10.52	86.38	9.42
18	400	110.60	6.17	83.36	9.40
	200	96.94	9.84	98.95	9.81
	20	99.16	9.57	96.26	12.07
19	300	88.37	8.76	83.94	8.74
	150	95.79	5.85	86.30	11.95
	10	103.73	7.60	102.13	4.23

ISSN: 1749-8546