Fig. 7
SGE blocked the activation of NF-κB in IL-1β-induced SW982 cells and joint muscles of CIA rats. SW982 cells were seeded in 6-well plates and confocal dish (5 × 105 cells/well) and allowed to grow for 24 h. Cells were pretreated with the indicated concentrations of SGE for 1 h, then stimulated with IL-1β (20 ng/mL) for 1 h or 12 h; on day 50, the joint muscles were separated from the hind paw of mice and lysed by cell lysis buffer. The expression of P-p65 and COX-2 in SW982 cells (a, b) and joint muscles (c, d) were detected by western-blotting. The translocation of p65 (e, f) was determined by immunofluorescence staining (###P < 0.001 vs. Ctrl group; ***P < 0.001 vs. IL-1β group or CIA group; n = 3 for the cell experiment and n = 6 for the animal experiment)