Fig. 6

Silencing of Atg7 enhanced cell apoptosis and AIF nuclear translocation under serum-starved condition. a The autophagy inhibition effect of siAtg7 was detected by western blot. b Flow cytometry was used to detect the apoptosis of cells transfected with sicontrol or siAtg7 in the presence or absence of serum. c The effect of knockdown siAtg7 in expression level of AIF in nuclear. HeLa and C-33A cells were transfected with sicontrol or siAtg7, after 24 h the cells were cultured in the presence or absence of serum for another 24 h, and then collected for nuclear fractionation. The expression of AIF was determined by western blotting